Anti-TLR7 Antibody (4T732) is a Rabbit antibody targeting TLR7. Anti-TLR7 Antibody (4T732) can be used in FCM,WB.

IgG

4T732

Human (predicted:Rat,Mouse)

1. Blank control: THP-1.
Primary Antibody (green line): Rabbit Anti-TLR7 antibody (TMAB-13575)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line) : Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Sample:
Lane 1: Recombinant human TLR7 protein, His
Primary: Anti-TLR7 (TMAB-13575) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 121 kDa
Observed band size: 40 kDa

FCM: 1μg/Test; WB: 1:500-2000

Monoclonal

Recombinant Protein: human TLR7 protein

Human

TLR7

Toll-like receptor 7

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.