Antibody Type: Recombinant Rabbit Monoclonal

Application: WB,FCM

Reactivity: Human,Mouse

IgG

7Y355

Human,Mouse

1. THP-1 (H) cells were treated with or without LPS (1 μg/ml) for 24 h, 25 μg total protein per Lane of cell lysates probed with TLR2 monoclonal antibody, unconjugated (TMAB-01853) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
2. The THP-1 (H) cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C,the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Rabbit Anti-TLR2 antibody (TMAB-01853): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Blank control (black): PBS.
3. The Raw264.7 (M) cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C,the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Rabbit Anti-TLR2 antibody (TMAB-01853): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Blank control (black): PBS.

WB,FCM

WB: 1:500-2000; FCM: 1ug/Test

Monoclonal

Recombinant Protein: full length protein of human TLR2

Human

7097

Inflammatory mediators,Receptors,Macrophage / Inflamm.,TLR Signaling,Defensin,Transmembrane