Anti-TLE1 Antibody (6Y958) is a Rabbit antibody targeting TLE1. Anti-TLE1 Antibody (6Y958) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG

6Y958

Human

1. Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TLE1 Monoclonal Antibody, Unconjugated (TMAB-13568) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TLE1 Monoclonal Antibody, Unconjugated (TMAB-13568) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. 25 μg total protein per lane of various lysates (see on figure) probed with TLE1 monoclonal antibody, unconjugated (TMAB-13568) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200

Monoclonal

A synthesized peptide: human TLE1

Human

TLE1

Transducin-like enhancer protein 1

Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.