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Anti-TIMP-1 Polyclonal Antibody 2
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Catalog No. TMAB-01845
Anti-TIMP-1 Polyclonal Antibody 2 is a Rabbit antibody targeting TIMP-1. Anti-TIMP-1 Polyclonal Antibody 2 can be used in WB,IHC-P.
Anti-TIMP-1 Polyclonal Antibody 2
Anti-TIMP-1 Polyclonal Antibody 2
😃Good
Catalog No. TMAB-01845
Anti-TIMP-1 Polyclonal Antibody 2 is a Rabbit antibody targeting TIMP-1. Anti-TIMP-1 Polyclonal Antibody 2 can be used in WB,IHC-P.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P Reactivity: Human |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Sample: Lane 1: TIMP-1 fusion protein 0.2 μg Primary: Anti-TIMP-1 (TMAB-01845) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kDa Observed band size: 25 kDa 2. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-TIMP-1 (CT) Polyclonal Antibody, Unconjugated (TMAB-01845) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Paraformaldehyde-fixed, paraffin embedded (human esophagus cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TIMP-1) Polyclonal Antibody, Unconjugated (TMAB-01845) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (human esophagus cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TIMP-1) Polyclonal Antibody, Unconjugated (TMAB-01845) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TIMP-1) Polyclonal Antibody, Unconjugated (TMAB-01845) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | WB,IHC-P |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq]. |
| Immunogen | KLH conjugated synthetic peptide: human TIMP-1 |
| Antigen Species | Human |
| Gene ID | 7076 |
| Uniprot ID | |
| Biology Area | TIMPs,TIMPs,TIMP,TIMPs,TIMP1 / TIMP2 |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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