Anti-TIE1 Polyclonal Antibody is a Rabbit antibody targeting TIE1. Anti-TIE1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human (predicted:Mouse,Rat)

1. Sample:
A549 (Human) Cell Lysate at 30 μg
HepG2 (Human) Cell Lysate at 30 μg
Primary: Anti-TIE1 (TMAB-01838) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 123 kDa
Observed band size: 123 kDa
2. Tissue/cell: human brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-TIE1 Polyclonal Antibody, Unconjugated (TMAB-01838) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Tie1

Human

Growth factor receptors,Endothelium,Mesoderm,Receptor Tyrosine Kinases,Endothelial Markers,Mesoderm