Anti-Thyroid peroxidase Polyclonal Antibody is a Rabbit antibody targeting Thyroid peroxidase. Anti-Thyroid peroxidase Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human,Rat (predicted:Mouse)

1. Tissue/cell: Rat brain; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Thyroid peroxidase Polyclonal Antibody, Unconjugated (TMAB-01837) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Lane 1: MCF-7 (Human) Cell Lysate at 30 μg
Lane 2: K562 (Human) Cell Lysate at 30 μg
Lane 3: A673 (Human) Cell Lysate at 30 μg
Lane 4: A431 (Human) Cell Lysate at 30 μg
Primary:
Anti-Thyroid peroxidase (TMAB-01837) at 1/1000 dilution
Anti-beta-Actin at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 101 kDa
Observed band size: 105 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Thyroid peroxidase

Human

TPO

Thyroid peroxidase

Cellular metabolic process,Hormone biosynthesis,Hormone biosynthesis,Antioxidants,Thyroid axis,Hormones

Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).