Anti-Thrombomodulin Polyclonal Antibody is a Rabbit antibody targeting Thrombomodulin. Anti-Thrombomodulin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Dog,Cow)

1. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Thrombomodulin Polyclonal Antibody, Unconjugated (TMAB-13521) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Lane 1: Testis (Mouse) Tissue Lysate at 40 μg
Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 μg
Lane 3: Testis (Rat) Tissue Lysate at 40 μg
Lane 4: A431 (Human) Cell Lysate at 30 μg
Lane 5: Huvec (Human) Cell Lysate at 30 μg
Lane 6: A549 (Human) Cell Lysate at 30 μg
Primary: Anti-Thrombomodulin (TMAB-13521) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 120 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: mouse Thrombomodulin

Mouse

THBD

Thrombomodulin

Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.