Anti-THRB Antibody (1H386) is a Mouse antibody targeting THRB. Anti-THRB Antibody (1H386) can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

1H386

Human,Mouse,Rat

1. Blank control (black line): HepG2.
Primary Antibody (green line): Mouse Anti-THRB antibody (TMAB-13515)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-Mouse IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Mouse IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (THRB) Monoclonal Antibody, Unconjugated (TMAB-13515) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (THRB) Monoclonal Antibody, Unconjugated (TMAB-13515) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining
4. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (THRB) Monoclonal Antibody, Unconjugated (TMAB-13515) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (THRB) Monoclonal Antibody, Unconjugated (TMAB-13515) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (mouse thyroid gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (THRB) Monoclonal Antibody, Unconjugated (TMAB-13515) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (Human thyroid gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (THRB) Monoclonal Antibody, Unconjugated (TMAB-13515) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
8. Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human A431 cell lysates
Primary: Anti-THRB (TMAB-13515) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:400-800; IF: 1:100-500; FCM: 1μg/Test

Monoclonal

Recombinant Protein: human THRB protein

Human

THRB

Thyroid hormone receptor beta

Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.