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Anti-THRA1+2 Polyclonal Antibody

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Catalog No. TMAB-13513

Anti-THRA1+2 Polyclonal Antibody is a Rabbit antibody targeting THRA1+2. Anti-THRA1+2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-THRA1+2 Polyclonal Antibody

Anti-THRA1+2 Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-13513
Anti-THRA1+2 Polyclonal Antibody is a Rabbit antibody targeting THRA1+2. Anti-THRA1+2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2217-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5297-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-THRA1+2 Polyclonal Antibody is a Rabbit antibody targeting THRA1+2. Anti-THRA1+2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse (predicted:Human,Rat,Chicken,Dog,Pig,Cow,Zebrafish,Sheep,Cat)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (THRA1+2) Polyclonal Antibody, Unconjugated (TMAB-13513) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample: Stomach (Mouse) Lysate at 40 μg
Primary: Anti-THRA1+2 (TMAB-13513) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 53 kD
3. Sample: Stomach (Mouse) Lysate at 40 μg
Primary: Anti-THRA1+2 (TMAB-13513) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
Tissue SpecificityAfter the mid-blastula transition (MBT), expressed throughout the deep cells, which give rise to the embryo proper. In adults, isoform 2 shows highest expression in the eye and liver. Expressed in adult gonads.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human THRA1 and THRA2
Antigen Species
Human
Gene Name
THRA
Gene ID
Protein Name
Thyroid hormone receptor alpha
Uniprot ID
Function
Nuclear hormone receptor. High affinity receptor for triiodothyronine.
Chemical Properties
Molecular WeightTheoretical: 55 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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