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Anti-Thioredoxin/TRX Antibody (1K412)

😃Good
Catalog No. TMAY-00733
Alias TXN, TRX1, TRX, TRDX, thioredoxin, RP11-427L11.1, MGC61975, DKFZp686B1993

Anti-Thioredoxin/TRX Antibody (1K412) is a Rabbit antibody targeting Thioredoxin/TRX. Anti-Thioredoxin/TRX Antibody (1K412) can be used in WB,IP.

Anti-Thioredoxin/TRX Antibody (1K412)

Anti-Thioredoxin/TRX Antibody (1K412)

😃Good
Catalog No. TMAY-00733Alias TXN, TRX1, TRX, TRDX, thioredoxin, RP11-427L11.1, MGC61975, DKFZp686B1993
Anti-Thioredoxin/TRX Antibody (1K412) is a Rabbit antibody targeting Thioredoxin/TRX. Anti-Thioredoxin/TRX Antibody (1K412) can be used in WB,IP.
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100 μL$269 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Thioredoxin/TRX Antibody (1K412) is a Rabbit antibody targeting Thioredoxin/TRX. Anti-Thioredoxin/TRX Antibody (1K412) can be used in WB,IP.
AliasTXN, TRX1, TRX, TRDX, thioredoxin, RP11-427L11.1, MGC61975, DKFZp686B1993
Ig Type
Monoclonal Rabbit IgG
Clone
1K412
Reactivity
Human
Verified Activity
1. Anti-TXN rabbit monoclonal antibody at 1:2000 dilution.

-Lane A: Hela Whole Cell Lysate.

-Lane B: THP-1 Whole Cell Lysate.

-Lane C: A431 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:12 kDa.

-Observed band size:14 kDa.

2. TXN was immunoprecipitated using:

-Lane A:0.5 mg Hela Whole Cell Lysate.

-Lane B:0.5 mg 293T Whole Cell Lysate.

-2 µL anti-TXN rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-TXN rabbit monoclonal antibody, at 1:100 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 13 kDa.

-Observed band size: 13 kDa
Application
WB,IP
Recommended Dose
WB: 1:2000-1:10000; IP: 4-6 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human Thioredoxin / TXN (rh Thioredoxin / TXN; TMPY-02250; P10599; Met1-Val105).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundThioredoxin, also known as ATL-derived factor, Surface-associated sulphydryl protein, SASP and TXN, is a nucleus, cytoplasm and secreted protein that belongs to the thioredoxin family. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxins are found in nearly all known organisms and are essential for life in mammals. Thioredoxin / TXN participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Thioredoxin / TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Thioredoxin / TXN nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Thioredoxin / TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human Thioredoxin / TXN protein (TMPY-02250)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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