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Anti-Thioredoxin 2 Polyclonal Antibody
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Catalog No. TMAB-13507
Anti-Thioredoxin 2 Polyclonal Antibody is a Rabbit antibody targeting Thioredoxin 2. Anti-Thioredoxin 2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Thioredoxin 2 Polyclonal Antibody
Anti-Thioredoxin 2 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-13507
Anti-Thioredoxin 2 Polyclonal Antibody is a Rabbit antibody targeting Thioredoxin 2. Anti-Thioredoxin 2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
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| Description | Anti-Thioredoxin 2 Polyclonal Antibody is a Rabbit antibody targeting Thioredoxin 2. Anti-Thioredoxin 2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Thioredoxin 2) Polyclonal Antibody, Unconjugated (TMAB-13507) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Sample: Lane 1: Mouse Kidney tissue lysates Lane 2: Mouse Small intestine tissue lysates Lane 3: Rat Cerebrum tissue lysates Lane 4: Rat Spleen tissue lysates Lane 5: Human K562 cell lysates Lane 6: Human HepG2 cell lysates Lane 7: Human 293T cell lysates Lane 8: Human Jurkat cell lysates Lane 9: Human HeLa cell lysates Primary: Anti-Thioredoxin 2 (TMAB-13507) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12 kDa Observed band size: 12 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Mitochondrion. |
| Tissue Specificity | Widely expressed in adult (at protein level) and fetal tissues. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide isomerase(PDI) and a number of transcription factors such as p53, NF-kB and AP-1. Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins. |
| Immunogen | KLH conjugated synthetic peptide: human Thioredoxin 2 |
| Antigen Species | Human |
| Gene Name | TXN2 |
| Gene ID | |
| Protein Name | Thioredoxin, mitochondrial |
| Uniprot ID | |
| Function | Has an anti-apoptotic function and plays an important role in the regulation of mitochondrial membrane potential. Could be involved in the resistance to anti-tumor agents. Possesses a dithiol-reducing activity. |
| Molecular Weight | Theoretical: 12 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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