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Anti-TFAM Polyclonal Antibody is a Rabbit antibody targeting TFAM. Anti-TFAM Polyclonal Antibody can be used in FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $372 | 7-10 days | |
200 μL | $528 | 7-10 days |
Description | Anti-TFAM Polyclonal Antibody is a Rabbit antibody targeting TFAM. Anti-TFAM Polyclonal Antibody can be used in FCM. |
Ig Type | IgG |
Reactivity | Human (predicted:Rat,Mouse) |
Verified Activity | Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-MtTF1/TFAM antibody (TMAB-01822) Dilution: 1 μg/Test; Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488 Dilution: 0.5 μg/Test. Isotype control (orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. |
Application | FCM |
Recommended Dose | FCM: 1ug/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Mitochondrion. Mitochondrion matrix, mitochondrion nucleoid. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M. |
Immunogen | KLH conjugated synthetic peptide: human MtTF1 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Transcription factors/regulators,Transcription Factors,Mitochondrial transcription,Mitochondrial Biogenesis,Mitochondrial markers,Mitochondrial,Mitochondria |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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