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Anti-TFAM Polyclonal Antibody

😃Good
Catalog No. TMAB-01822

Anti-TFAM Polyclonal Antibody is a Rabbit antibody targeting TFAM. Anti-TFAM Polyclonal Antibody can be used in FCM.

Anti-TFAM Polyclonal Antibody

Anti-TFAM Polyclonal Antibody

😃Good
Catalog No. TMAB-01822
Anti-TFAM Polyclonal Antibody is a Rabbit antibody targeting TFAM. Anti-TFAM Polyclonal Antibody can be used in FCM.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$372 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Anti-TFAM Polyclonal Antibody is a Rabbit antibody targeting TFAM. Anti-TFAM Polyclonal Antibody can be used in FCM.
Ig Type
IgG
Reactivity
Human (predicted:Rat,Mouse)
Verified Activity
Blank control (black line): Hela.
Primary Antibody (green line): Rabbit Anti-MtTF1/TFAM antibody (TMAB-01822)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
FCM
Recommended Dose
FCM: 1ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMitochondrion. Mitochondrion matrix, mitochondrion nucleoid.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundBinds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human MtTF1
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Transcription factors/regulators,Transcription Factors,Mitochondrial transcription,Mitochondrial Biogenesis,Mitochondrial markers,Mitochondrial,Mitochondria
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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