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Anti-Testin Polyclonal Antibody

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Catalog No. TMAB-13459

Anti-Testin Polyclonal Antibody is a Rabbit antibody targeting Testin. Anti-Testin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Testin Polyclonal Antibody

Anti-Testin Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-13459
Anti-Testin Polyclonal Antibody is a Rabbit antibody targeting Testin. Anti-Testin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2207-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5287-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-Testin Polyclonal Antibody is a Rabbit antibody targeting Testin. Anti-Testin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Dog,Pig,Rabbit,Sheep)
Verified Activity
1. Sample: Lung (Mouse) Lysate at 40 μg
Primary: Anti-Testin (TMAB-13459) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
2. Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Testin Polyclonal Antibody, Unconjugated (TMAB-13459) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Cell junction, focal adhesion.Note=Detected along actin stress fibers.
Tissue SpecificityUbiquitous.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundCancer-associated chromosomal changes often involveregions containing fragile sites. This gene maps to a commomfragile site on chromosome 7q31.2 designated FRA7G. This gene issimilar to mouse Testin, a testosterone-responsive gene encoding aSertoli cell secretory protein containing three LIM domains. LIMdomains are double zinc-finger motifs that mediate protein-proteininteractions between transcription factors, cytoskeletal proteinsand signaling proteins. This protein is a negative regulator ofcell growth and may act as a tumor suppressor. This scaffoldprotein may also play a role in cell adhesion, cell spreading andin the reorganization of the actin cytoskeleton. Multiple proteinisoforms are encoded by transcript variants of this gene.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Testin
Antigen Species
Human
Gene Name
TES
Gene ID
Protein Name
Testin
Uniprot ID
Function
Scaffold protein that may play a role in cell adhesion,cell spreading and in the reorganization of the actin cytoskeleton.Plays a role in the regulation of cell proliferation. May act as atumor suppressor. Inhibits tumor cell growth.
Chemical Properties
Molecular WeightTheoretical: 48 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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