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Anti-TBX3 Antibody (5E363)

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Catalog No. TMAB-13414

Anti-TBX3 Antibody (5E363) is a Rabbit antibody targeting TBX3. Anti-TBX3 Antibody (5E363) can be used in WB,IP,FCM,ICC/IF.

Anti-TBX3 Antibody (5E363)

Anti-TBX3 Antibody (5E363)

Copy Product Info
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Catalog No. TMAB-13414
Anti-TBX3 Antibody (5E363) is a Rabbit antibody targeting TBX3. Anti-TBX3 Antibody (5E363) can be used in WB,IP,FCM,ICC/IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1497-10 days7-10 days
50 μL$2647-10 days7-10 days
100 μL$4737-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-TBX3 Antibody (5E363) is a Rabbit antibody targeting TBX3. Anti-TBX3 Antibody (5E363) can be used in WB,IP,FCM,ICC/IF.
Ig Type
IgG
Clone
5E363
Reactivity
Human (predicted:Mouse,Rat)
Verified Activity
1. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (XTBX3) monoclonal Antibody, unconjugated (TMAB-13414) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
2. 25 μg total protein per lane of various lysates (see on figure) probed with TBX3 monoclonal antibody, unconjugated (TMAB-13414) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
Application
Recommended Dose
WB: 1:500-2000; IP: 1:20-50; FCM: 1:50-100; ICC/IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus (Potential).
Tissue SpecificityWidely expressed.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundTranscriptional repressor involved in developmental processes.Binds to the palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence, or a half-site, which are present in the regulatory region of several genes. Probably plays a role in limb pattern formation.
Antigen Details
Immunogen
A synthesized peptide: human TBX3
Antigen Species
Human
Gene Name
TBX3
Gene ID
Protein Name
T-box transcription factor TBX3
Uniprot ID
Function
Transcriptional repressor involved in developmental processes. Probably plays a role in limb pattern formation.
Chemical Properties
Molecular WeightTheoretical: 79 kDa. Actual: 85 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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