Anti-TBX18 Polyclonal Antibody is a Rabbit antibody targeting TBX18. Anti-TBX18 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,Rabbit)

1. Sample：
line1: Mouse embryo lysate at 45 ug;
line2: Mouse brain lysate at 45 ug;
Primary: Anti-TBX18 (TMAB-13407) at 1: 200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG at 1: 3000 dilution;
Predicted band size: 65 kD
Observed band size: 67 kD
We are unsure as to the identity of these extra bands
2. Sample: HT29 Cell lysate at 30 μg;
Primary: Anti-TBX18 (TMAB-13407) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1: 5000 dilution;
Predicted band size: 65 kD
Observed band size: 65 kD
3. Paraformaldehyde-fixed, paraffin embedded (mouse embryo); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TBX18) Polyclonal Antibody, Unconjugated (TMAB-13407) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human TBX18

Human

TBX18

T-box transcription factor TBX18

Probable transcriptional regulator involved in developmental processes.