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Anti-TBR2 Polyclonal Antibody

😃Good
Catalog No. TMAB-01812
Alias T-brain-2, Tbr 2, T-box brain protein 2, t box brain 2, Eomesodermin homolog, Eomesodermin, EOMES

Anti-TBR2 Polyclonal Antibody is a Rabbit antibody targeting TBR2. Anti-TBR2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

Anti-TBR2 Polyclonal Antibody

Anti-TBR2 Polyclonal Antibody

😃Good
Catalog No. TMAB-01812Alias T-brain-2, Tbr 2, T-box brain protein 2, t box brain 2, Eomesodermin homolog, Eomesodermin, EOMES
Anti-TBR2 Polyclonal Antibody is a Rabbit antibody targeting TBR2. Anti-TBR2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3727-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-TBR2 Polyclonal Antibody is a Rabbit antibody targeting TBR2. Anti-TBR2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
AliasT-brain-2, Tbr 2, T-box brain protein 2, t box brain 2, Eomesodermin homolog, Eomesodermin, EOMES
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Chicken,Cow,Horse,Sheep)
Verified Activity
1. Blank control (Black line): Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-TBR2antibody (TMAB-01812)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-TBR2 Polyclonal Antibody, Unconjugated (TMAB-01812) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
Application
IHC-P,IHC-Fr,IF,FCM
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundEOMES (eomesodermin homolog), also known as TBR2 (T-box-brain2), is the 686 amino acid human homolog of the mouse Eomes protein that contains one T-box DNA binding domain. Genes that contain T-box domains encode proteins that function as transcription factors and are often involved in the regulation of various developmental events. Localized to the nucleus and expressed in the developing brain, EOMES is thought to be involved in neuronal migration and division and may play a role in trophoblast development and gastrulation. Silencing of the EOMES gene can cause mutated or arrested development and may lead to microcephaly disorders which are characterized by reduced head circumference and a malformed brain.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human TBR2
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Trophectoderm,Germ cell markers,Soma marker,Neural Signal Transduction,Neurogenesis,Embryonic Germ Cells,Intracellular
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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