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Anti-TBR2 Polyclonal Antibody is a Rabbit antibody targeting TBR2. Anti-TBR2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $372 | 7-10 days | |
200 μL | $529 | 7-10 days |
Description | Anti-TBR2 Polyclonal Antibody is a Rabbit antibody targeting TBR2. Anti-TBR2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM. |
Alias | T-brain-2, Tbr 2, T-box brain protein 2, t box brain 2, Eomesodermin homolog, Eomesodermin, EOMES |
Ig Type | IgG |
Reactivity | Human,Rat (predicted:Mouse,Chicken,Cow,Horse,Sheep) |
Verified Activity | 1. Blank control (Black line): Molt4 (Black). Primary Antibody (green line): Rabbit Anti-TBR2antibody (TMAB-01812) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-TBR2 Polyclonal Antibody, Unconjugated (TMAB-01812) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
Application | IHC-P,IHC-Fr,IF,FCM |
Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Nucleus. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | EOMES (eomesodermin homolog), also known as TBR2 (T-box-brain2), is the 686 amino acid human homolog of the mouse Eomes protein that contains one T-box DNA binding domain. Genes that contain T-box domains encode proteins that function as transcription factors and are often involved in the regulation of various developmental events. Localized to the nucleus and expressed in the developing brain, EOMES is thought to be involved in neuronal migration and division and may play a role in trophoblast development and gastrulation. Silencing of the EOMES gene can cause mutated or arrested development and may lead to microcephaly disorders which are characterized by reduced head circumference and a malformed brain. |
Immunogen | KLH conjugated synthetic peptide: human TBR2 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Trophectoderm,Germ cell markers,Soma marker,Neural Signal Transduction,Neurogenesis,Embryonic Germ Cells,Intracellular |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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