Anti-TAPA1/CD81 Antibody (3G748) is a Rabbit antibody targeting TAPA1/CD81. Anti-TAPA1/CD81 Antibody (3G748) can be used in WB,FCM,ICC/IF.

IgG

3G748

Human,Mouse,Rat

1. 25 μg total protein per lane of various lysates (see on figure) probed with TAPA1/CD81 monoclonal antibody, unconjugated (TMAB-13377) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. 25 μg total protein per lane of various lysates (see on figure) probed with TAPA1/CD81 monoclonal antibody, unconjugated (TMAB-13377) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
3. The THP-1 (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-TAPA1/CD81 antibody (TMAB-13377): 1 μg/10^6 cells; Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
4. 4% Paraformaldehyde-fixed THP-1 (H) cell; Antibody incubation with (TAPA1/CD81) monoclonal Antibody, unconjugated (TMAB-13377) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

WB: 1:500-2000; FCM: 1μg/Test; ICC/IF: 1:50-200

Monoclonal

A synthesized peptide: human CD81

Human

CD81

CD81 antigen

May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV.