Anti-TAF3 Antibody (8H232) is a Rabbit antibody targeting TAF3. Anti-TAF3 Antibody (8H232) can be used in FCM, ICC/IF, IF, IHC-Fr, IHC-P.

IgG

8H232

Human

1. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-TAF3 antibody (TMAB-13362, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
2. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TAF3 Monoclonal Antibody, Unconjugated (TMAB-13362) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TAF3 Monoclonal Antibody, Unconjugated (TMAB-13362) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.

FCM=1:50-100; ICC/IF=1:50-200; IF=1:100-200; IHC-Fr=1:100-200; IHC-P=1:100-200

Monoclonal

A synthesized peptide: human TAF3

Human

TAF3

Transcription initiation factor TFIID subunit 3