Anti-SYTL5 Polyclonal Antibody is a Rabbit antibody targeting SYTL5. Anti-SYTL5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse,Rabbit)

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-SYTL5 Polyclonal Antibody, Unconjugated (TMAB-13345) 1: 200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1: 200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml, blue) was used to stain the cell nuclei
2. Sample:
Lane 1: Human MCF-7 cell lysates
Lane 2: Human U251 cell lysates
Lane 3: Human HeLa cell lysates
Lane 4: Human A549 cell lysates
Primary: Anti-SYTL5 (TMAB-13345) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 81 kDa
Observed band size: 75 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human SYTL5

Human

SYTL5

Synaptotagmin-like protein 5

May act as Rab effector protein and play a role in vesicle trafficking. Binds phospholipids.