Anti-Syntaxin 1A Antibody (3B814) is a Rabbit antibody targeting Syntaxin 1A. Anti-Syntaxin 1A Antibody (3B814) can be used in WB,IP,FCM.

IgG

3B814

Human,Mouse,Rat

1. Western blot analysis of SH-SY5Y cell lysate. Using Syntaxin 1A (TMAB-13335) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The SH-SY5Y (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-Syntaxin 1A antibody (TMAB-13335, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:1000-2000; IP: 1:20-50; FCM: 1:50-100

Monoclonal

A synthesized peptide: human Syntaxin 1A

Human

STX1A

Syntaxin-1A

Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.