Anti-SYNPR/Synaptoporin Polyclonal Antibody is a Rabbit antibody targeting SYNPR/Synaptoporin. Anti-SYNPR/Synaptoporin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Rat (predicted:Human,Mouse)

1. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SYNPR) Polyclonal Antibody, Unconjugated (TMAB-13331) at 1:800 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
2. Blank control (blue): RSC96 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-SYNPR/Synaptoporin antibody (TMAB-13331), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA.
3. Sample: Bulbus olfactorius (Rat) Lysate at 40 μg
Primary: Anti-SynaptoporiN (TMAB-13331) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 28 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human Synaptoporin

Human

SYNPR

Synaptoporin

Intrinsic membrane protein of small synaptic vesicles. Probable vesicular channel protein.