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Anti-Syndecan-1 Polyclonal Antibody
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Catalog No. TMAB-01804
Anti-Syndecan-1 Polyclonal Antibody is a Rabbit antibody targeting Syndecan-1. Anti-Syndecan-1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Syndecan-1 Polyclonal Antibody
Anti-Syndecan-1 Polyclonal Antibody
😃Good
Catalog No. TMAB-01804
Anti-Syndecan-1 Polyclonal Antibody is a Rabbit antibody targeting Syndecan-1. Anti-Syndecan-1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse,Rat,Cow (predicted:Chicken,Dog,Pig,Horse,Rabbit) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat,Cow (predicted:Chicken,Dog,Pig,Horse,Rabbit) |
| Verified Activity | 1. Sample: Lane 1: Mouse Kidney tissue lysates Lane 2: Mouse Liver tissue lysates Lane 3: Human U251 cell lysates Lane 4: Human A431 cell lysates Lane 5: Human HepG2 cell lysates Lane 6: Human HeLa cell lysates Primary: Anti-Syndecan-1 (TMAB-01804) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kDa Observed band size: 74 kDa 2. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Syndecan-1) Polyclonal Antibody, Unconjugated (TMAB-01804) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Syndecan-1) Polyclonal Antibody, Unconjugated (TMAB-01804) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Syndecan-1) Polyclonal Antibody, Unconjugated (TMAB-01804) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass type I membrane protein. Secreted. Note=Shedding of the ectodomain produces a soluble form. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human CD138 |
| Antigen Species | Human |
| Gene ID | 6382 |
| Uniprot ID | |
| Biology Area | Metabolism of lipids and lipoproteins,Extracellular matrix,Lipid metabolism,Cancer,ECM Proteins,Membrane Proteins,Synapse marker,Neurogenesis,Glucosaminoglycans,HSPGs,B Lymphocytic Lineage |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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