Anti-Synaptotagmin XII Polyclonal Antibody is a Rabbit antibody targeting Synaptotagmin XII. Anti-Synaptotagmin XII Polyclonal Antibody can be used in WB,FCM.

IgG

Human (predicted:Mouse,Rat,Dog,Pig,Rabbit,Sheep)

1. Sample: U-2Os (Human) Cell Lysate at 30 μg
Primary: Anti-Synaptotagmin XII (TMAB-13323) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 48 kD
2. Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-Synaptotagmin XII antibody (TMAB-13323)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg /test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human Synaptotagmin XII

Human

SYT12

Synaptotagmin-12

May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis.