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Anti-Synapsin-1 Polyclonal Antibody

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Catalog No. TMAB-01801

Anti-Synapsin-1 Polyclonal Antibody is a Rabbit antibody targeting Synapsin-1. Anti-Synapsin-1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Synapsin-1 Polyclonal Antibody

Anti-Synapsin-1 Polyclonal Antibody

😃Good
Catalog No. TMAB-01801
Anti-Synapsin-1 Polyclonal Antibody is a Rabbit antibody targeting Synapsin-1. Anti-Synapsin-1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$373 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF

Reactivity: Human,Rat (predicted:Mouse,Pig)
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Pig)
Verified Activity
1. Sample:
A549 (Human) Cell Lysate at 40 μg
Primary: Anti-Synapsin 1 (TMAB-01801) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDa
2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (Synapsin 1) Polyclonal Antibody, Unconjugated (TMAB-01801) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
3. Sample:
HL-60 (Human) Cell Lysate at 40 μg
Primary: Anti-Synapsin 1 (TMAB-01801) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDa
4. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Synapsin 1) Polyclonal Antibody, Unconjugated (TMAB-01801) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell junction, synapse. Golgi apparatus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein encoded serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations in this gene may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Synapsin I
Antigen Species
Human
Gene ID
6853
Uniprot ID
Biology Area
Synapse marker,SNAPs & SNAREs
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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