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Anti-SV2A Polyclonal Antibody

😃Good
Catalog No. TMAB-01799
Alias Synaptic vesicle glycoprotein 2A, SV2A, p87

Anti-SV2A Polyclonal Antibody is a Rabbit antibody targeting SV2A. Anti-SV2A Polyclonal Antibody can be used in FCM.

Anti-SV2A Polyclonal Antibody

Anti-SV2A Polyclonal Antibody

😃Good
Catalog No. TMAB-01799Alias Synaptic vesicle glycoprotein 2A, SV2A, p87
Anti-SV2A Polyclonal Antibody is a Rabbit antibody targeting SV2A. Anti-SV2A Polyclonal Antibody can be used in FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3747-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-SV2A Polyclonal Antibody is a Rabbit antibody targeting SV2A. Anti-SV2A Polyclonal Antibody can be used in FCM.
AliasSynaptic vesicle glycoprotein 2A, SV2A, p87
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit)
Verified Activity
1. Black line: Positive blank control (Molt4); Negative blank control (A549)
Green line: Primary Antibody (Rabbit Anti-SV2A antibody (TMAB-01799))
Orange line: Isotype Control Antibody (Rabbit IgG).
Blue line: Secondary Antibody (Goat anti-rabbit IgG-AF488)/(Goat anti-rabbit IgG-AF647)
Molt4 (Positive) and A549 (Negative control) cells (black) were fixed with 4% PFA for 10 min at room temperature, permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SV2A Antibody (TMAB-01799) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody (blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
2. Blank control: Molt-4. Primary Antibody (green line): Rabbit Anti-SV2A antibody
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 20% PBST for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
FCM
Recommended Dose
FCM: 1ug/test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundSV2s (Synaptic Vesicle protein 2) are integral membrane glycoproteins present in all synaptic vesicles. They have 12 transmembrane domains predicted by sequence analysis. There are three characterized isoforms, SV2A, SV2B and SV2C. SV2A is expressed ubiquitously throughout the brain. SV2B has a more restricted distribution with varying degrees of coexpression with SV2A. SV2C is more closely related to SV2A but shows a very restricted expression pattern. The highest expression levels were observed in phylogenetically old brain areas like pallidum, the midbrain and the olfactory bulb. SV2A is probably involved in the maintenance of a pool of synaptic vesicles competent for calcium-stimulated exocytosis. SV2A is a highly glycosylated synaptic vesicle protein with homology to transmembrane transporters.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human SV2A
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
More Ion Channels,Calcium Pump,Synapse marker
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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