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Anti-Sulfiredoxin-1 Polyclonal Antibody
😃Good
Catalog No. TMAB-01797
Anti-Sulfiredoxin-1 Polyclonal Antibody is a Rabbit antibody targeting Sulfiredoxin-1. Anti-Sulfiredoxin-1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Sulfiredoxin-1 Polyclonal Antibody
Anti-Sulfiredoxin-1 Polyclonal Antibody
😃Good
Catalog No. TMAB-01797
Anti-Sulfiredoxin-1 Polyclonal Antibody is a Rabbit antibody targeting Sulfiredoxin-1. Anti-Sulfiredoxin-1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Sample: Kidney (Mouse) Lysate at 40 μg Primary: Anti-SRXN1 (TMAB-01797) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (secondary antibody) at 1/5000 dilution Predicted band size: 15 kDa Observed band size: 15 kDa 2. Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 μg Lane 2: Cerebrum (Rat) Lysate at 40 μg Lane 3: HepG2 (Human) Cell Lysate at 30 μg Lane 4: A549 (Human) Cell Lysate at 30 μg Primary: Anti-SRXN1 (TMAB-01797) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15 kDa Observed band size: 15 kDa 3. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by microwave in sodium citratebuffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RTfor 30 min; Antibody incubation with (SRXN1) Polyclonal/MonoclonalAntibody, Unconjugated (TMAB-01797) at 1:400 overnight at 4°C,followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:50-200 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasmic |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Sulfiredoxin, also designated Sulfiredoxin-1 and chromosome 20 open reading frame 139 (C20orf139), is a cytoplasmic antioxidant protein involved in signaling through catalytic reduction of oxidative modifications. It regulates peroxiredoxins (PRXs), a family of proteins that reduce hydroperoxides, by reducing the conserved cysteine from sulfinic to sulfenic acid. This impacts the role of PRX in the reduction of other downstream transcription factors and kinase signaling pathways. The Sulfiredoxin protein specifically acts on the PRDX1, PRDX2, PRDX3 and PRDX4 peroxiredoxins, but not on PRDX5 or PRDX6. Sulfiredoxin acts as a phosphotransferase and an athioltransferase and is widely expressed, with highest levels detected in lung, spleen, kidney and thymus tissues. |
| Immunogen | KLH conjugated synthetic peptide: human SRXN1 |
| Antigen Species | Human |
| Gene ID | 140809 |
| Uniprot ID | |
| Biology Area | Oxidative Stress,Oxidative stress |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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