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Anti-STUB1 Antibody (5M430) (Synonyms: STUB1, STIP1 Homology and U Box-Containing Protein 1, E3 Ubiquitin-Prot...)

Catalog No. TMAC-03909 Copy Product Info
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Anti-STUB1 Antibody (5M430) is a Rabbit antibody targeting STUB1. Anti-STUB1 Antibody (5M430) can be used in FCM,ICC,IF,IHC,IP,WB.

Anti-STUB1 Antibody (5M430)

Copy Product Info
🥰Excellent
Catalog No. TMAC-03909
Synonyms STUB1, STIP1 Homology and U Box-Containing Protein 1, E3 Ubiquitin-Protein Ligase CHIP, CLL-Associated Antigen KW-8, CHIP, Carboxy Terminus of Hsp70-Interacting Protein, Antigen NY-CO-7

Anti-STUB1 Antibody (5M430) is a Rabbit antibody targeting STUB1. Anti-STUB1 Antibody (5M430) can be used in FCM,ICC,IF,IHC,IP,WB.

Anti-STUB1 Antibody
(5M430)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2977-10 days7-10 days
100 μL$4987-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-STUB1 Antibody (5M430) is a Rabbit antibody targeting STUB1. Anti-STUB1 Antibody (5M430) can be used in FCM,ICC,IF,IHC,IP,WB.
SynonymsSTUB1, STIP1 Homology and U Box-Containing Protein 1, E3 Ubiquitin-Protein Ligase CHIP, CLL-Associated Antigen KW-8, CHIP, Carboxy Terminus of Hsp70-Interacting Protein, Antigen NY-CO-7
Ig Type
IgG
Clone
5M430
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of STUB1 on different lysates using anti-STUB1 antibody at 1/2,000 dilution. Positive control: Lane 1: 293, Lane 2: MCF-7, Lane 3: SK-Br-3, Lane 4: Mouse kidney.
2. Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-STUB1 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-STUB1 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-STUB1 antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-STUB1 antibody. Counter stained with hematoxylin.
6. ICC staining STUB1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining STUB1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. ICC staining STUB1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
9. Flow cytometric analysis of SH-SY-5Y cells with STUB1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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Application
Recommended Dose
WB: 1:1000-2000; IHC: 1:50-200; ICC: 1:50-200; IP: 1:10-50; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundCHIP (carboxy terminus of HSP 70-interacting protein), also designated STIP1 homology and U-box containing protein 1, HSPABP2, NY-CO-7, SDCCAG7 and STUB1, is a cytoplasmic E3 ubiquitin ligase that influences protein ubiquitylation. CHIP interacts with Smad1/Smad4 and blocks BMP signaling through the ubiquitin-mediated degradation of Smad proteins. CHIP controls both association of Hsp70/Hsp90 chaperones with ErbB2 and down-regulation of ErbB2 induced by inhibitors of Hsp90. A 1.3-kb transcript is most abundant in striated muscle (heart and skeletal muscle), with lower expression in pancreas and brain.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: human STUB1 aa 1-200
Antigen Species
human
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 35 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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