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Anti-STUB1 Antibody (5M430)
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Catalog No. TMAC-03909
Alias STUB1, STIP1 Homology and U Box-Containing Protein 1, E3 Ubiquitin-Protein Ligase CHIP, CLL-Associated Antigen KW-8, CHIP, Carboxy Terminus of Hsp70-Interacting Protein, Antigen NY-CO-7
Anti-STUB1 Antibody (5M430) is a Rabbit antibody targeting STUB1. Anti-STUB1 Antibody (5M430) can be used in WB,ICC,IF,IHC,IP,FCM.
Anti-STUB1 Antibody (5M430)
Anti-STUB1 Antibody (5M430)
😃Good
Catalog No. TMAC-03909Alias STUB1, STIP1 Homology and U Box-Containing Protein 1, E3 Ubiquitin-Protein Ligase CHIP, CLL-Associated Antigen KW-8, CHIP, Carboxy Terminus of Hsp70-Interacting Protein, Antigen NY-CO-7
Anti-STUB1 Antibody (5M430) is a Rabbit antibody targeting STUB1. Anti-STUB1 Antibody (5M430) can be used in WB,ICC,IF,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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Product Introduction
Bioactivity
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Antigen Details
Chemical Properties
| Description | Anti-STUB1 Antibody (5M430) is a Rabbit antibody targeting STUB1. Anti-STUB1 Antibody (5M430) can be used in WB,ICC,IF,IHC,IP,FCM. |
| Synonyms | STUB1, STIP1 Homology and U Box-Containing Protein 1, E3 Ubiquitin-Protein Ligase CHIP, CLL-Associated Antigen KW-8, CHIP, Carboxy Terminus of Hsp70-Interacting Protein, Antigen NY-CO-7 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of STUB1 on different lysates using anti-STUB1 antibody at 1/2,000 dilution. Positive control: Lane 1: 293, Lane 2: MCF-7, Lane 3: SK-Br-3, Lane 4: Mouse kidney. 2. Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-STUB1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-STUB1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-STUB1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-STUB1 antibody. Counter stained with hematoxylin. 6. ICC staining STUB1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining STUB1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining STUB1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of SH-SY-5Y cells with STUB1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC: 1:50-200; IP: 1:10-50; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | CHIP (carboxy terminus of HSP 70-interacting protein), also designated STIP1 homology and U-box containing protein 1, HSPABP2, NY-CO-7, SDCCAG7 and STUB1, is a cytoplasmic E3 ubiquitin ligase that influences protein ubiquitylation. CHIP interacts with Smad1/Smad4 and blocks BMP signaling through the ubiquitin-mediated degradation of Smad proteins. CHIP controls both association of Hsp70/Hsp90 chaperones with ErbB2 and down-regulation of ErbB2 induced by inhibitors of Hsp90. A 1.3-kb transcript is most abundant in striated muscle (heart and skeletal muscle), with lower expression in pancreas and brain. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: human STUB1 aa 1-200 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 35 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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