Anti-STRAP Antibody (5Y959) is a Mouse antibody targeting STRAP. Anti-STRAP Antibody (5Y959) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG1, Kappa

5Y959

Human

1. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:5000
Primary ab incubation condition: room temperature 2 h
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: HeLa, 2: 293
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 38 kDa
Observed MW: 38 kDa
2. Tissue: Human colon
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-13244
3. Tissue: Human-lung-squamous-carcinoma
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-13244

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500

Monoclonal

KLH conjugated synthetic peptide: human STRAP

Human

STRAP

Serine-threonine kinase receptor-associated protein

The SMN complex plays an essential role in spliceosomalsnRNP assembly in the cytoplasm and is required for pre-mRNAsplicing in the nucleus. STRAP may play a role in the cellulardistribution of the SMN complex. Negatively regulates TGF-betasignaling but positively regulates the PDPK1 kinase activity byenhancing its autophosphorylation and by significantly reducing theassociation of PDPK1 with 14-3-3 protein.