Anti-STK38 Polyclonal Antibody is a Rabbit antibody targeting STK38. Anti-STK38 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Horse,GuineaPig)

1. Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-STK38 Polyclonal Antibody, Unconjugated (TMAB-13231) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Spleen (Mouse) Lysate at 40 μg
Lung (Mouse) Lysate at 40 μg
Thymus (Mouse) Lysate at 40 μg
Testis (Mouse) Lysate at 40 μg
A431 (Human) Cell Lysate at 30 μg
Primary: Anti-STK38 (TMAB-13231) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human STK38

Human

STK38

Serine/threonine-protein kinase 38

Negative regulator of MAP3K1/2 signaling. Converts MAP3K2 from its phosphorylated form to its nonphosphorylated form and inhibits autophosphorylation of MAP3K2.