Anti-STIP1 Polyclonal Antibody is a Rabbit antibody targeting STIP1. Anti-STIP1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse)

1. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (STIP1) Polyclonal Antibody, Unconjugated (TMAB-13218) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (STIP1) Polyclonal Antibody, Unconjugated (TMAB-13218) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (STIP1) Polyclonal Antibody, Unconjugated (TMAB-13218) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (STIP1) Polyclonal Antibody, Unconjugated (TMAB-13218) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human STIP1

Human

STIP1

Stress-induced-phosphoprotein 1

SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.