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Anti-Staufen Antibody (9G746) is a Rabbit antibody targeting Staufen. Anti-Staufen Antibody (9G746) can be used in WB,ICC/IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $296 | 7-10 days | |
100 μL | $496 | 7-10 days |
Description | Anti-Staufen Antibody (9G746) is a Rabbit antibody targeting Staufen. Anti-Staufen Antibody (9G746) can be used in WB,ICC/IF,FCM. |
Synonyms | staufen-like, Staufen, RNA binding protein, homolog 1 (Drosophila), Staufen, Drosophila, homolog of, 1, Staufen RNA binding protein homolog 1, Staufen RNA binding protein (Drosophila), staufen, STAU1_HUMAN, STAU1, STAU, PPP1R150, MGC124588, FLJ25010, Double-stranded RNA-binding protein Staufen homolog 1, Double stranded RNA binding protein Staufen homolog 1, Double stranded RNA binding protein Staufen homolog |
Ig Type | IgG |
Clone | 9G746 |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of Staufen on K562 cell using anti-Staufen antibody at 1/1,000 dilution. 2. ICC staining Staufen in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining Staufen in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining Staufen in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of SH-SY5Y cells with Staufen antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Application | |
Recommended Dose | WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1:50-100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | STAU1 (staufen, RNA binding protein, homolog 1) is a 577 amino acid protein that contains three double-stranded RNA-binding domains and is a mammalian homolog of Staufen, a Drosophila protein that is involved in mRNA transport during oogenesis and zygotic development. Localized to the rough endoplasmic reticulum (RER) and expressed in a variety of tissues, including heart, brain, liver, lung, pancreas, kidney and placenta, STAU1 binds to both Tubulin and double-stranded RNA and is thought to play an important role in mRNA transport from the microtubule network to the RER. Additionally, STAU1 may be involved in cross-linking cytoskeletal components with RNA, an event that is important for proper mRNA positioning during translation. Alternative splicing of the STAU1 gene yields two STAU1 isoforms, designated short and long. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 63 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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