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Anti-STAT5A/B Antibody (8D628) (Synonyms: Transcription factor STAT5B, Transcription factor STAT5A, STAT5B, S...)
Catalog No. TMAC-03863 Copy Product Info
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Anti-STAT5A/B Antibody (8D628) is a Rabbit antibody targeting STAT5A/B. Anti-STAT5A/B Antibody (8D628) can be used in FCM,ICC/IF,IHC,IP,WB.
Anti-STAT5A/B Antibody (8D628)
Copy Product Info🥰Excellent
Catalog No. TMAC-03863
Synonyms Transcription factor STAT5B, Transcription factor STAT5A, STAT5B, STAT5, STAT 5B, STAT 5A, STA5A_HUMAN, Signal Transducer and Activator of Transcription 5B, Signal transducer and activator of transcription 5A, MGF
Anti-STAT5A/B Antibody (8D628) is a Rabbit antibody targeting STAT5A/B. Anti-STAT5A/B Antibody (8D628) can be used in FCM,ICC/IF,IHC,IP,WB.
Anti-STAT5A/B Antibody
(8D628)
(8D628)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $296 | 7-10 days | 7-10 days | |
| 100 μL | $497 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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| Description | Anti-STAT5A/B Antibody (8D628) is a Rabbit antibody targeting STAT5A/B. Anti-STAT5A/B Antibody (8D628) can be used in FCM,ICC/IF,IHC,IP,WB. |
| Synonyms | Transcription factor STAT5B, Transcription factor STAT5A, STAT5B, STAT5, STAT 5B, STAT 5A, STA5A_HUMAN, Signal Transducer and Activator of Transcription 5B, Signal transducer and activator of transcription 5A, MGF |
| Ig Type | IgG |
| Clone | 8D628 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of STAT5A+B on different lysates using anti-STAT5A+B antibody at 1/1,000 dilution. Positive control: Lane 1: K562, Lane 2: Jurkat, Lane 3: PC-12. 2. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-STAT5A+B antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-STAT5A+B antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-STAT5A+B antibody. Counter stained with hematoxylin. 5. ICC staining STAT5A+B in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining STAT5A+B in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining STAT5A+B in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of NIH/3T3 cells with STAT5A+B antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.         |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Stat5 (Signal Transducers and Activators of Transcription 5) is important in regulating T cell functions involving the receptors for Interleukin-2 (IL-2). IL-2 stimulates the rapid phosphorylation of both serine and tyrosine residues of Stat5a and Stat5b in human T lymphocytes and in several IL-2-responsive lymphocytic cell lines. IL-2 differentially induces serine phosphorylation of Stat5a and Stat5b on Ser726 and Ser731, respectively. Stat5b is preferentially phosphorylated and displays more protracted serine phosphorylation kinetics than Stat5a. Both the acid-rich region and the COOH terminus of IL-2Rb can independently mediate IL-2-induced Stat 5a/b serine phosphorylation, suggesting that Stat5a/b serine phosphorylation occurs at a postreceptor level. Stat5a is phosphorylated on Tyr694 in a prolactin-sensitive manner, whereas serine phosphorylation is constitutive. Activation of Stat5 by IL-2 may help govern the biological effects of IL-2 during the immune response. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Molecular Weight | Theoretical: 90 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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