Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF,FCM

Reactivity: Human,Mouse,Rat (predicted:Dog,Cow,Horse)

IgG

Human,Mouse,Rat (predicted:Dog,Cow,Horse)

1. Blank control (blue line): Hela (fixed with 80% methanol (5 min at-20°C) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
Primary Antibody (green line): Rabbit Anti-StAR antibody (TMAB-01780), Dilution: 3 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 μg/test.
2. Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (StAR) Polyclonal Antibody, Unconjugated (TMAB-01780) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
3. Sample:
Lane 1: Ovary (Mouse) Lysate at 40 μg
Lane 2: Cerebrum (Mouse) Lysate at 40 μg
Lane 3: Kidney (Mouse) Lysate at 40 μg
Lane 4: Cerebrum (Rat) Lysate at 40 μg
Lane 5: K562 (Human) Cell Lysate at 30 μg
Lane 6: THP-1 (Human) Cell Lysate at 30 μg
Lane 7: U937 (Human) Cell Lysate at 30 μg
Lane 8: HepG2 (Human) Cell Lysate at 30 μg
Primary:
Anti-StAR (TMAB-01780) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 28 kDa

WB,IHC-P,IHC-Fr,IF,FCM

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 3μg /Test

Polyclonal

KLH conjugated synthetic peptide: human StAR/StARD1

Human

6770

Hormone biosynthesis,Metabolism of lipids and lipoproteins,Hormone biosynthesis,Lipid metabolism,Mitochondrial markers,Hormones,Mitochondrial,Organelle Proteins