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Anti-SQLE Polyclonal Antibody is a Rabbit antibody targeting SQLE. Anti-SQLE Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $528 | 7-10 days |
Description | Anti-SQLE Polyclonal Antibody is a Rabbit antibody targeting SQLE. Anti-SQLE Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
Reactivity | Human,Rat (predicted:Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit) |
Verified Activity | 1. Sample: NIH/3T3 (Mouse) Cell Lysate at 30 μg A549 (Human) Cell Lysate at 30 μg Primary: Anti-Squalene Epoxidase (TMAB-01768) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kDa Observed band size: 64 kDa 2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Squalene Epoxidase Polyclonal Antibody, Unconjugated (TMAB-01768) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | This protein catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway. |
Immunogen | KLH conjugated synthetic peptide: human Squalene Epoxidase |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Metabolism of lipids and lipoproteins,Lipid metabolism |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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