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Anti-SQLE Polyclonal Antibody

(Synonyms: Squalene monooxygenase, Squalene epoxidase (SE), SQLE, ERG1) Copy Product Info
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Synonyms: Squalene monooxygenase, Squalene epoxidase (SE), SQLE, ERG1

Catalog No. TMAB-01768 Copy Product Info
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Anti-SQLE Polyclonal Antibody is a Rabbit antibody targeting SQLE. Anti-SQLE Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

Anti-SQLE Polyclonal Antibody

Pack SizePriceUSA StockGlobal StockQuantity
50 µL$2227-10 days7-10 days
100 µL$3747-10 days7-10 days
200 µL$5287-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-SQLE Polyclonal Antibody is a Rabbit antibody targeting SQLE. Anti-SQLE Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
SynonymsSqualene monooxygenase, Squalene epoxidase (SE), SQLE, ERG1
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit)
Verified Activity
1. Sample:
NIH/3T3 (Mouse) Cell Lysate at 30 μg
A549 (Human) Cell Lysate at 30 μg
Primary: Anti-Squalene Epoxidase (TMAB-01768) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Squalene Epoxidase Polyclonal Antibody, Unconjugated (TMAB-01768) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
Anti-SQLE Polyclonal Antibody,Anti-SQLE Polyclonal Antibody
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMicrosome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1 mg/mL
Research BackgroundThis protein catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Squalene Epoxidase
Antigen Species
Human
Gene Name
SQLE
Gene ID
Protein Name
Squalene monooxygenase
Uniprot ID
Biology Area
Metabolism of lipids and lipoproteins,Lipid metabolism
Function
Catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway.
Chemical Properties
Molecular WeightTheoretical: 64 kDa. Actual: 64 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: Anti-SQLE Polyclonal Antibody molecular weight