Anti-SQLE Polyclonal Antibody is a Rabbit antibody targeting SQLE. Anti-SQLE Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human,Rat (predicted:Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit)

1. Sample:
NIH/3T3 (Mouse) Cell Lysate at 30 μg
A549 (Human) Cell Lysate at 30 μg
Primary: Anti-Squalene Epoxidase (TMAB-01768) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Squalene Epoxidase Polyclonal Antibody, Unconjugated (TMAB-01768) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Squalene Epoxidase

Human

SQLE

Squalene monooxygenase

Metabolism of lipids and lipoproteins,Lipid metabolism

Catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway.