Anti-SPT5 Antibody (4X309) is a Rabbit antibody targeting SPT5. Anti-SPT5 Antibody (4X309) can be used in FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB.

IgG

4X309

Human (predicted:Mouse,Rat)

1. 25 ug total protein per lane of various lysates (see on figure) probed with SPT5 monoclonal antibody, unconjugated (TMAB-13141) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-SPT5 antibody (TMAB-13141, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
3. 4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (SPT5) monoclonal Antibody, unconjugated (TMAB-13141) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

FCM=1:50-100; ICC/IF=1:50-200; IF=1:50-200; IHC-Fr=1:100-200; IHC-P=1:100-200; WB=1:500-2000

Monoclonal

A synthesized peptide: human SPT5

Human

SUPT5H

Transcription elongation factor SPT5