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Anti-Sphingosine Kinase 1/SPHK1 Polyclonal Antibody
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Catalog No. TMAB-01766
Anti-Sphingosine Kinase 1/SPHK1 Polyclonal Antibody is a Rabbit antibody targeting Sphingosine Kinase 1/SPHK1. Anti-Sphingosine Kinase 1/SPHK1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Sphingosine Kinase 1/SPHK1 Polyclonal Antibody
Anti-Sphingosine Kinase 1/SPHK1 Polyclonal Antibody
😃Good
Catalog No. TMAB-01766
Anti-Sphingosine Kinase 1/SPHK1 Polyclonal Antibody is a Rabbit antibody targeting Sphingosine Kinase 1/SPHK1. Anti-Sphingosine Kinase 1/SPHK1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Mouse,Rat (predicted:Human,Dog,Cow,Rabbit) |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Dog,Cow,Rabbit) |
| Verified Activity | 1. Sample: Heart (Mouse) Lysate at 30 μg Primary: Anti-SPHK1 (TMAB-01766) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa 2. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SPHK1) Polyclonal Antibody, Unconjugated (TMAB-01766) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 3. Sample: Lane 1: Mouse Pancreas tissue lysates Lane 2: Mouse Lung tissue lysates Lane 3: Rat Kidney tissue lysates Primary: Anti-SPHK1 (TMAB-01766) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42-52 kDa Observed band size: 50 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| Immunogen | KLH conjugated synthetic peptide: human SPHK1 |
| Antigen Species | Human |
| Gene ID | 8877 |
| Uniprot ID | |
| Biology Area | Metabolism of lipids and lipoproteins,Lipid metabolism,GPCR,Lipid Kinases |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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