Anti-Sphingosine Kinase 1/SPHK1 Polyclonal Antibody is a Rabbit antibody targeting Sphingosine Kinase 1/SPHK1. Anti-Sphingosine Kinase 1/SPHK1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Mouse,Rat (predicted:Human,Dog,Cow,Rabbit)

1. Sample: Heart (Mouse) Lysate at 30 μg
Primary: Anti-SPHK1 (TMAB-01766) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
2. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SPHK1) Polyclonal Antibody, Unconjugated (TMAB-01766) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Sample:
Lane 1: Mouse Pancreas tissue lysates
Lane 2: Mouse Lung tissue lysates
Lane 3: Rat Kidney tissue lysates
Primary: Anti-SPHK1 (TMAB-01766) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42-52 kDa
Observed band size: 50 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human SPHK1

Human

SPHK1

Sphingosine kinase 1

Metabolism of lipids and lipoproteins,Lipid metabolism,GPCR,Lipid Kinases

Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.