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Anti-SPESP1 Polyclonal Antibody
Catalog No. TMAB-13104 Copy Product Info
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Anti-SPESP1 Polyclonal Antibody is a Rabbit antibody targeting SPESP1. Anti-SPESP1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-SPESP1 Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-13104
Anti-SPESP1 Polyclonal Antibody is a Rabbit antibody targeting SPESP1. Anti-SPESP1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-SPESP1 Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
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| Description | Anti-SPESP1 Polyclonal Antibody is a Rabbit antibody targeting SPESP1. Anti-SPESP1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human (predicted:Rat,Rabbit) |
| Verified Activity | Paraformaldehyde-fixed, paraffin embedded (human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SPESP1) Polyclonal Antibody, Unconjugated (TMAB-13104) at 1:800 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasmic vesicle > secretory vesicle > acrosome. Small proacrosomal granules (during the Golgi phase), enlarged acrosomal vesicles (during the cap phase), acrosome (during the elongating phase), equatorial segment of the acrosome. |
| Tissue Specificity | Highly expressed in testis, where it is localized in the acrosome of postmeiotic stages of spermiogenesis (round and elongating spermatids and in ejaculated spermatozoa) (at protein level). Poorly expressed in placenta and fetal lung. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | SPESP1 is a 399 amino acid protein belonging to the SPESP1 family. Localizing to cytoplasmic vesicle, secretory vesicle, and acrosome, SPESP1 is highly expressed in testis, with lower levels found in placenta and fetal lung. SPESP1 establishes an equatorial segment subcompartment early in sperm development and is required for proper sperm-egg fusion. Disruption of SPESP1 leads to abnormal distribution of sperm proteins resulting in a detached membrane from the equatorial segment and less fertile sperm. SPESP1 may interact with IZUMO1 and MN9 antigen and contains an N-glycosylation site as well as several cAMP-dependent kinase, protein kinase C, and casein kinase II consensus phosphorylation sites. |
| Immunogen | KLH conjugated synthetic peptide: human SPESP1 |
| Antigen Species | Human |
| Gene Name | SPESP1 |
| Gene ID | |
| Protein Name | Sperm equatorial segment protein 1 |
| Uniprot ID | |
| Function | The encoded protein is a human alloantigen involved in sperm-egg binding and fusion. [provided by RefSeq, Apr 2010] |
| Molecular Weight | Theoretical: 37 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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