Anti-Spectrin alpha chain Antibody (1D833) is a Rabbit antibody targeting Spectrin alpha chain. Anti-Spectrin alpha chain Antibody (1D833) can be used in WB,ICC/IF,FCM.

IgG

1D833

Human,Mouse (predicted:Rat)

1. 25 ug total protein per lane of various lysates (see on figure) probed with Spectrin alpha chain monoclonal antibody, unconjugated (TMAB-13099) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The K562 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-Spectrin alpha chain antibody (TMAB-13099, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1:50-100

Monoclonal

A synthesized peptide: human Spectrin alpha chain

Human

SPTA1

Spectrin alpha chain, erythrocytic 1

Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.