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Anti-Spastin Polyclonal Antibody
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Catalog No. TMAB-13081
Anti-Spastin Polyclonal Antibody is a Rabbit antibody targeting Spastin. Anti-Spastin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Spastin Polyclonal Antibody
Anti-Spastin Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-13081
Anti-Spastin Polyclonal Antibody is a Rabbit antibody targeting Spastin. Anti-Spastin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
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| Description | Anti-Spastin Polyclonal Antibody is a Rabbit antibody targeting Spastin. Anti-Spastin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Sample: Cerebrum (Mouse) Lysate at 40 μg Heart (Mouse) Lysate at 40 μg Cerebrum (Rat) Lysate at 40 μg Primary: Anti-Spastin (TMAB-13081) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 68 kD 2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-FSP/Spastin Polyclonal Antibody, Unconjugated (TMAB-13081) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 3. Sample: Lane 1: Human SH-SY5Y cell lysates Lane 2: Human Molt-4 cell lysates Lane 3: Human HL60 cell lysates Lane 4: Human Huvec cell lysates Primary: Anti-Spastin (TMAB-13081) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 60 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass membrane protein (Potential). Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Endoplasmic reticulum. Endosome. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Localization to the centrosome is independent of microtubules. Localizes to the midbody of dividing cells, and this requires CHMP1B. Enriched in the distal axons and branches of postmitotic neurons. Isoform 3 is the main endosomal form. |
| Tissue Specificity | Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. The short isoforms may predominate in brain and spinal cord. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | This gene encodes a member of the AAA (ATPases associated with a variety of cellular activities) protein family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. The encoded ATPase may be involved in the assembly or function of nuclear protein complexes. Two transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described but their full length sequences have not been determined. Mutations associated with this gene cause the most frequent form of autosomal dominant spastic paraplegia 4. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human FSP |
| Antigen Species | Human |
| Gene Name | SPAST |
| Gene ID | |
| Protein Name | Spastin |
| Uniprot ID |
| Molecular Weight | Theoretical: 68 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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