Anti-SPAG4L/SRG4 Polyclonal Antibody is a Rabbit antibody targeting SPAG4L/SRG4. Anti-SPAG4L/SRG4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human)

1. Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-SRG4/TSARG4 Polyclonal Antibody, Unconjugated (TMAB-13077) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: mouse stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-SRG4/TSARG4 Polyclonal Antibody, Unconjugated (TMAB-13077) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Sample: Brain (Mouse) Lysate at 30 μg
Primary: Anti-SPAG4L (TMAB-13077) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1: 5000 dilution;
Predicted band size: 42 kD
Observed band size: 42 kD
4. Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-SPAG4L Polyclonal Antibody, Unconjugated (TMAB-13077) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human TSARG4

Human

SUN5

SUN domain-containing protein 5