Anti-SOX10 Polyclonal Antibody is a Rabbit antibody targeting SOX10. Anti-SOX10 Polyclonal Antibody can be used in WB,FCM.

IgG

Mouse,Rat (predicted:Human,Dog,Pig,Cow,Rabbit,Sheep)

1. Blank control: B16.
Primary Antibody (green line): Rabbit Anti-SOX10 antibody (TMAB-13052)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. 25 μg total protein per lane of various lysates (see on figure) probed with SOX10 polyclonal antibody, unconjugated (TMAB-13052) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human SOX10

Human

SOX10

Transcription factor SOX-10

Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia.