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Anti-Sodium Potassium ATPase Antibody (8O42) (Synonyms: sodium-potassium ATPase catalytic subunit alpha-1, Sodi...)

Catalog No. TMAB-01753 Copy Product Info
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Anti-Sodium Potassium ATPase Antibody (8O42) is a Mouse antibody targeting Sodium Potassium ATPase. Anti-Sodium Potassium ATPase Antibody (8O42) can be used in IF,IHC-Fr,IHC-P,WB.

Anti-Sodium Potassium ATPase Antibody (8O42)

Copy Product Info
🥰Excellent
Catalog No. TMAB-01753
Synonyms sodium-potassium ATPase catalytic subunit alpha-1, Sodium/potassium-transporting ATPase subunit alpha-1, Sodium pump subunit alpha-1, Sodium pump 1, Sodium potassium ATPase alpha 1 polypeptide, Nkaa1b, Na+/K+ATPase alpha 1 subunit, Na+/K+ATPase 1, Na+, K+ATPase alpha subunit, Na, K ATPase alpha 1 subunit, Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase 1, Na K ATPase catalytic subunit alpha A protein, Na K ATPase alpha A catalytic polypeptide, MGC51750, MGC38419, MGC3285, EC 3.6.3.9, BC010319, ATPase Na+/K+transporting subunit alpha 1, ATPase Na+/K+transporting alpha 1 polypeptide, Atpa-1, ATP1A1, AT1A1, AT1A 1, alpha 1 Sodium Potassium ATPase, A1A1

Anti-Sodium Potassium ATPase Antibody (8O42) is a Mouse antibody targeting Sodium Potassium ATPase. Anti-Sodium Potassium ATPase Antibody (8O42) can be used in IF,IHC-Fr,IHC-P,WB.

Anti-Sodium Potassium ATPase Antibody
(8O42)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2207-10 days7-10 days
100 μL$3737-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Bioactivity
Description
Anti-Sodium Potassium ATPase Antibody (8O42) is a Mouse antibody targeting Sodium Potassium ATPase. Anti-Sodium Potassium ATPase Antibody (8O42) can be used in IF,IHC-Fr,IHC-P,WB.
Synonymssodium-potassium ATPase catalytic subunit alpha-1, Sodium/potassium-transporting ATPase subunit alpha-1, Sodium pump subunit alpha-1, Sodium pump 1, Sodium potassium ATPase alpha 1 polypeptide, Nkaa1b, Na+/K+ATPase alpha 1 subunit, Na+/K+ATPase 1, Na+, K+ATPase alpha subunit, Na, K ATPase alpha 1 subunit, Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase 1, Na K ATPase catalytic subunit alpha A protein, Na K ATPase alpha A catalytic polypeptide, MGC51750, MGC38419, MGC3285, EC 3.6.3.9, BC010319, ATPase Na+/K+transporting subunit alpha 1, ATPase Na+/K+transporting alpha 1 polypeptide, Atpa-1, ATP1A1, AT1A1, AT1A 1, alpha 1 Sodium Potassium ATPase, A1A1
Ig Type
IgG1, κ
Clone
8O42
Reactivity
Human,Mouse,Rat
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
13. Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
14. Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
15. Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
16. Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
17. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
18. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
19. 25 μg total protein per Lane of various lysates probed with Sodium Potassium ATPase monoclonal antibody, unconjugated (TMAB-01753) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
20. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
21. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
22. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
verifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivity
Application
IF
IHC-Fr
IHC-P
WB
Recommended Dose
WB: 1:2000-10000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationCell membrane; Multi-pass membrane protein. Melanosome.
Tissue SpecificityFound in most tissues.
ConstructionHybridoma Monoclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1 mg/mL
Research BackgroundThe protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+-ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May2009].
Antigen Details
Immunogen
Recombinant Protein: human Sodium Potassium ATPase
Antigen Species
Human
Gene Name
ATP1A1
Gene ID
476
Protein Name
Sodium/potassium-transporting ATPase subunit alpha-1
Uniprot ID
P05023
Function
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
Chemical Properties
Molecular WeightTheoretical: 113 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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