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Anti-Sodium Potassium ATPase Antibody (8O42) is a Mouse antibody targeting Sodium Potassium ATPase. Anti-Sodium Potassium ATPase Antibody (8O42) can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $373 | 7-10 days |
Description | Antibody Type: Mouse Monoclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse,Rat |
Ig Type | IgG1,k |
Clone | 8O42 |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 11. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 12. Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 13. Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 14. Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 15. Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 16. Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 17. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 18. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 19. 25 μg total protein per Lane of various lysates probed with Sodium Potassium ATPase monoclonal antibody, unconjugated (TMAB-01753) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 20. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. 21. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. 22. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:2000-10000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Subcellular Localization | Cell membrane; Multi-pass membrane protein. Melanosome. |
Construction | Hybridoma Monoclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+-ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May2009]. |
Immunogen | Recombinant Protein: human Sodium Potassium ATPase |
Antigen Species | Human |
Gene ID | 476 |
Uniprot ID |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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