Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF

Reactivity: Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit)

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit)

1. Sample:
Brain (Rat) lysate at 30 μg;
Liver (Rat) lysate at 30 μg;
Primary: Anti-NHE1 (TMAB-01752) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit Igg (bse-0295G) at 1: 3000 dilution;
Predicted band size: 91 kDa
Observed band size: 91 kDa
We are unsure as to the identity of these extra bands
2. Sample:
molt-4 (human) cell Lysate at 40 μg
Primary: Anti-Sodium hydrogen exchanger 1 (TMAB-01752) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 91 kDa
Observed band size: 91 kDa
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Sodium hydrogen exchanger 1) Polyclonal Antibody, Unconjugated (TMAB-01752) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human NHE1

Human

6548

Sodium/ion transport,Cancer,Heart disease,Transmembrane,Channels