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Anti-SOD2 Antibody (3S444)

Catalog No. TMAB-13028 Copy Product Info
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Anti-SOD2 Antibody (3S444) is a Mouse antibody targeting SOD2. Anti-SOD2 Antibody (3S444) can be used in WB,IHC-P,IHC-Fr,IF.

Anti-SOD2 Antibody (3S444)

Copy Product Info
🥰Excellent
Catalog No. TMAB-13028

Anti-SOD2 Antibody (3S444) is a Mouse antibody targeting SOD2. Anti-SOD2 Antibody (3S444) can be used in WB,IHC-P,IHC-Fr,IF.

Anti-SOD2 Antibody
(3S444)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2957-10 days7-10 days
100 μL$4737-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-SOD2 Antibody (3S444) is a Mouse antibody targeting SOD2. Anti-SOD2 Antibody (3S444) can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG1, k
Clone
3S444
Reactivity
Human,Mouse
Verified Activity
1. Sample:
Lane 1: Hela (Human) Cell Lysate at 30 μg
Lane 2: 293T (Human) Cell Lysate at 30 μg
Lane 3: Raw264.7 (Mouse) Cell Lysate at 30 μg
Primary: Anti-SOD2 (TMAB-13028) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 22 kD
2. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SOD2) Monoclonal Antibody, Unconjugated (TMAB-13028) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SOD2) Monoclonal Antibody, Unconjugated (TMAB-13028) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (Human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SOD2) Monoclonal Antibody, Unconjugated (TMAB-13028) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationMitochondrion matrix.
ConstructionHybridoma Monoclonal Antibody
PurificationProtein G purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
Recombinant Protein: human SOD protein
Antigen Species
Human
Gene Name
SOD2
Gene ID
Protein Name
Superoxide dismutase [Mn], mitochondrial
Uniprot ID
Function
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
Chemical Properties
Molecular WeightTheoretical: 22 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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