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Anti-SOD1 Polyclonal Antibody is a Rabbit antibody targeting SOD1. Anti-SOD1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-SOD1 Polyclonal Antibody is a Rabbit antibody targeting SOD1. Anti-SOD1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
Alias | superoxide dismutase 1, soluble, SOD, IPOA, hSod1, homodimer, HEL-S-44, ALS1, ALS |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat (predicted:Pig,Cow,Horse) |
Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SOD1) Polyclonal Antibody, Unconjugated (TMAB-01749) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane 1: Liver (Mouse) Lysate at 40 μg Lane 2: Cerebrum (Rat) Lysate at 40 μg Lane 3: Kidney (Rat) Lysate at 40 μg Lane 4: Liver (Rat) Lysate at 40 μg Primary: Anti-SOD1 (TMAB-01749) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kDa Observed band size: 19 kDa 3. Blank control: Jurkat. Primary Antibody (green line): Rabbit Anti-SOD1 antibody (TMAB-01749) Dilution: 1 μg/Test; Secondary Antibody: Goat anti-rabbit IgG-FITC Dilution: 0.5 μg/Test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 4. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-SOD1 Polyclonal Antibody, Unconjugated (TMAB-01749) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 5. Blank control: Raji. Primary Antibody (green line): Rabbit Anti-SOD1 antibody (TMAB-01749) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. |
Application | WB,IHC-P,IHC-Fr,IF,FCM |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cytoplasm. Note=The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008] |
Immunogen | KLH conjugated synthetic peptide: human SOD1 |
Antigen Species | Human |
Gene ID | |
Uniprot ID |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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