Antibody Type: Mouse Monoclonal

Application: WB, FCM, IP

Reactivity: Human

Mouse IgG2b

8C907

Human

1. Flow cytometric analysis of Human SOD1 expression in HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and stained with Purified Mouse anti-SOD1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
2. Anti-SOD1 mouse monoclonal antibody at 1:500 dilution.
-Lane A: Jurkat Whole Cell Lysate.
-Lane B: Hela Whole Cell Lysate.
-Lane C: HepG2 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:16 kDa.
-Observed band size:20 kDa.
3. SOD1 was immunoprecipitated using:
-Lane A:0.5 mg Jurkat Whole Cell Lysate.
-Lane B:0.5 mg HepG2 Whole Cell Lysate
-0.5 µL anti-SOD1 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-SOD1 mouse monoclonal antibody, at 1:500 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 16 kDa.
-Observed band size: 18 kDa

WB,FCM,IP

WB: 1:500-1:1000; FCM: 1:100-1:500; IP: 0.2-1 μL/mg of lysate

Monoclonal

Unconjugated

FITC/PE

3

Recombinant Protein: Human SOD1 / Superoxide Dismutase protein (TMPY-01585)

Human