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Anti-SOD1 Antibody (7V772)

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Catalog No. TMAB-13027

Anti-SOD1 Antibody (7V772) is a Rabbit antibody targeting SOD1. Anti-SOD1 Antibody (7V772) can be used in WB,IHC-P,IF,IHC-Fr.

Anti-SOD1 Antibody (7V772)

Anti-SOD1 Antibody (7V772)

Copy Product Info
😃Good
Catalog No. TMAB-13027
Anti-SOD1 Antibody (7V772) is a Rabbit antibody targeting SOD1. Anti-SOD1 Antibody (7V772) can be used in WB,IHC-P,IF,IHC-Fr.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1507-10 days7-10 days
50 μL$2627-10 days7-10 days
100 μL$4717-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-SOD1 Antibody (7V772) is a Rabbit antibody targeting SOD1. Anti-SOD1 Antibody (7V772) can be used in WB,IHC-P,IF,IHC-Fr.
Ig Type
IgG
Clone
7V772
Reactivity
Human,Mouse,Rat
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (SOD1) Monoclonal Antibody, Unconjugated (TMAB-13027) at 1:300 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (SOD1) Monoclonal Antibody, Unconjugated (TMAB-13027) at 1:300 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (SOD1) Monoclonal Antibody, Unconjugated (TMAB-13027) at 1:300 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (SOD1) Monoclonal Antibody, Unconjugated (TMAB-13027) at 1:300 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (SOD1) Monoclonal Antibody, Unconjugated (TMAB-13027) at 1:300 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
6. Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Liver tissue lysates
Lane 3: Mouse Placenta tissue lysates
Lane 4: Rat Cerebrum tissue lysates
Lane 5: Rat Placenta tissue lysates
Lane 6: Human HepG2 cell lysates
Lane 7: Human HeLa cell lysates
Lane 8: Human Jurkat cell lysates
Lane 9: Human MCF-7 cell lysates
Primary: Anti-SOD1 (TMAB-13027) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 15 kDa
Application
Recommended Dose
WB: 1:500-1000; IHC-P: 1:100-500; IF: 1:100-500; IHC-Fr: 1:100-500
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Note=The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
FormulationPBS, Glycerol, BSA.
Concentration1mg/ml
Research BackgroundThe protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human SOD1
Antigen Species
Human
Gene Name
SOD1
Gene ID
Protein Name
Superoxide dismutase [Cu-Zn]
Uniprot ID
Function
Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
Chemical Properties
Molecular WeightTheoretical: 17 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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