Anti-SOCS3 Polyclonal Antibody is a Rabbit antibody targeting SOCS3. Anti-SOCS3 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Sample:
Lane 1: Heart (Mouse) Lysate at 40 μg
Lane 2: Kidney (Mouse) Lysate at 40 μg
Lane 3: Cerebrum (Rat) Lysate at 40 μg
Lane 4: LympHnode (Rat) Lysate at 40 μg
Primary: Anti-SOCS3 (TMAB-01748) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kDa
Observed band size: 26 kDa
2. Blank control: U937. Primary Antibody (green line): Rabbit Anti-SOCS3 antibody (TMAB-01748)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB,FCM

WB: 1:500-2000; FCM: 1ug/Tes

Polyclonal

KLH conjugated synthetic peptide: human SOCS3

Human

Response to hypoxia,STATs,IL6 Related,Hypoxia,Obesity,Cytoplasmic,Intracellular Molecules