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Anti-SNAIL + SLUG Polyclonal Antibody
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Catalog No. TMAB-01747
Alias Zinc finger protein SNAI2, Zinc finger protein SNAI1, Zinc finger protein SLUG, WS2D, WS 2D, Snail2, SNAIL1, SNAIL+SLUG, Snail homolog 2, Snail homolog 1(Drosophila), Snail 2, SNAIL, SNAI2, SNAI1, SNAI 2, SNAH, Sna protein, SNA, SLUGH2, SLUGH1, SLUGH 1, SLUGH, Slug(chicken homolog) zinc finger protein, Slug zinc finger protein, Slug homolog zinc finger protein, Protein snail homolog 2, Protein snail homolog 1, Protein snail homolog, Protein sna, Neural crest transcription factor Slug, dJ710H13.1
Anti-SNAIL + SLUG Polyclonal Antibody is a Rabbit antibody targeting SNAIL + SLUG. Anti-SNAIL + SLUG Polyclonal Antibody can be used in WB,FCM.
Anti-SNAIL + SLUG Polyclonal Antibody
Anti-SNAIL + SLUG Polyclonal Antibody
😃Good
Catalog No. TMAB-01747Alias Zinc finger protein SNAI2, Zinc finger protein SNAI1, Zinc finger protein SLUG, WS2D, WS 2D, Snail2, SNAIL1, SNAIL+SLUG, Snail homolog 2, Snail homolog 1(Drosophila), Snail 2, SNAIL, SNAI2, SNAI1, SNAI 2, SNAH, Sna protein, SNA, SLUGH2, SLUGH1, SLUGH 1, SLUGH, Slug(chicken homolog) zinc finger protein, Slug zinc finger protein, Slug homolog zinc finger protein, Protein snail homolog 2, Protein snail homolog 1, Protein snail homolog, Protein sna, Neural crest transcription factor Slug, dJ710H13.1
Anti-SNAIL + SLUG Polyclonal Antibody is a Rabbit antibody targeting SNAIL + SLUG. Anti-SNAIL + SLUG Polyclonal Antibody can be used in WB,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-SNAIL + SLUG Polyclonal Antibody is a Rabbit antibody targeting SNAIL + SLUG. Anti-SNAIL + SLUG Polyclonal Antibody can be used in WB,FCM. |
| Alias | Zinc finger protein SNAI2, Zinc finger protein SNAI1, Zinc finger protein SLUG, WS2D, WS 2D, Snail2, SNAIL1, SNAIL+SLUG, Snail homolog 2, Snail homolog 1(Drosophila), Snail 2, SNAIL, SNAI2, SNAI1, SNAI 2, SNAH, Sna protein, SNA, SLUGH2, SLUGH1, SLUGH 1, SLUGH, Slug(chicken homolog) zinc finger protein, Slug zinc finger protein, Slug homolog zinc finger protein, Protein snail homolog 2, Protein snail homolog 1, Protein snail homolog, Protein sna, Neural crest transcription factor Slug, dJ710H13.1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Rabbit,Sheep,GuineaPig,Danio rerio) |
| Verified Activity | 1. Blank control (blue line): Hela (fixed with 70% methanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C). Primary Antibody (green line): Rabbit Anti-SNAIL + SLUG antibody (TMAB-01747), Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 μg/test. 2. Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-SNAIL+SLUG antibody (TMAB-01747) Dilution: 2 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488 Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 3. Heart Cell (mouse) Lysate at 40 μg Primary: Anti-SNAIL + SLUG (TMAB-01747) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kDa Observed band size: 30 kDa 4. Sample: Heart Cell (Rat) Lysate at 40 μg Primary: Anti-SNAIL + SLUG (TMAB-01747) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kDa Observed band size: 30 kDa 5. Sample: H9C2 Cell (Rat) Lysate at 30 μg HUVEC Cell (Human) Lysate at 30 μg MAD-MB-231 Cell (Human) Lysate at 30 μg 293T Cell (Human) Lysate at 30 μg Primary: Anti-SNAIL + SLUG (TMAB-01747) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kDa Observed band size: 30 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; FCM: 1μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Cytoplasm. Note=Observed in discrete foci in interphase nuclei. These nuclear foci do not overlap with the nucleoli, the SP100 and the HP1 heterochromatin or the coiled body, suggesting SNAI2 is associated with active transcription or active splicing regions. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. |
| Immunogen | KLH conjugated synthetic peptide: human SNAIL + SLUG |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID |
| Molecular Weight | Theoretical: 30 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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