Anti-SMURF2 Antibody (1Q728) is a Rabbit antibody targeting SMURF2. Anti-SMURF2 Antibody (1Q728) can be used in WB.

IgG

1Q728

Human (predicted:Mouse,Rat)

25 μg total protein per lane of various lysates (see on figure) probed with SMURF2 monoclonal antibody, unconjugated (TMAB-12987) at 1: 2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000

Monoclonal

A synthesized peptide: human SMURF2

Human

SMURF2

E3 ubiquitin-protein ligase SMURF2

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.